EC Number   |
|---|
   3.2.2.19 | - |
| 3.2.2.19 | 20000 fold by DE-52, phenyl-Sepharose, hydroxylapatite, Affi-Gel 501 organo-mercurial agarose, Ultrogel AcA 54 |
| 3.2.2.19 | 200000fold by DE-52, phenyl-Sepharose, hydroxylapatite, Ultrogel AcA 54, Mono Q chromatography |
| 3.2.2.19 | 800fold by DE-52, phenyl-Sepharose, carboxymethylcellulose, organomercurial agarose, Ultrogel AcA 54 |
| 3.2.2.19 | as a GST-fusion protein almost to homogeneity by affinity-column chromatography of glutathione-Sepharose |
| 3.2.2.19 | depending on the procedure used for purification, either a thiol-resistant or a thiol-sensitive species can be isolated. Organomercurial chromatography apparently results in the formation of the thiol-sensitive species of hydrolase as the exposure of the thiol-resistant form to HgCl2 does |
| 3.2.2.19 | homogenate of chicken muscle cells from tissue culture was centrifugated and the supernatant dialyzed |
| 3.2.2.19 | partial |
| 3.2.2.19 | partial by successive chromatography on DE-52 cellulose, phenyl-Sepharose and organomercurial agarose |
| 3.2.2.19 | recombinant His-tagged hARH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration |
