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Purification (Commentary):
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EC Number
Purification (Commentary)
3.1.6.2
-
3.1.6.2
99.3fold
3.1.6.2
affinity chromatography with NiCl2-primed HisTrap Chelating columns and concentrated 6-fold using ICON concentrators
3.1.6.2
enzyme does not copurify with oestrone sulfate sulfohydrolase
3.1.6.2
of the recombinant protein
3.1.6.2
partial
3.1.6.2
partial, the soluble enzyme
3.1.6.2
recombinant protein
3.1.6.2
solubilized with 0.5% Triton X-100, two fractions with cholesterol sulfate sulfohydrolase activity are separated by DEAE-cellulose chromatography, purification of the 30 kDa enzyme
3.1.6.2
to homogeneity
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