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EC Number
Purification (Commentary)
3.1.4.45
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3.1.4.45
499fold: recombinant UCE expressed in CHO-K1 cells, 1392fold: recombinant UCE expressed in Sf9 insect cells
3.1.4.45
affinity chromatography on HPC4 monoclonal antibody column
3.1.4.45
recombinant enzyme with TEV cleavage site and His6-tag by nickel affinity chromatography, tag cleavage by tobacco etch virus protease, another step of nickel affinity chromatography, and centrifugal ultrafiltration
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