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Search Purification (Commentary)

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Results 1 - 7 of 7
EC Number Purification (Commentary) Reference
Display the reaction diagram Show all sequences 3.1.3.72- 441601, 663772
Display the reaction diagram Show all sequences 3.1.3.72in absence of Triton X-100, very little enzyme activity is extracted from seeds 441604
Display the reaction diagram Show all sequences 3.1.3.72partial purification, extraction with 0.1% Triton X-100 increases recovery by nearly 4fold 94791
Display the reaction diagram Show all sequences 3.1.3.72partially purified, 6.5fold, by gel filtration 681220
Display the reaction diagram Show all sequences 3.1.3.72recombinant non-glycosylated His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant glycosylated nontagged enzyme from Pichia pastoris strain GS115(his4) by cation exchange chromatography 751936
Display the reaction diagram Show all sequences 3.1.3.72recovery is most complete when detergent or phospholipase C is included in extracting medium 94801
Display the reaction diagram Show all sequences 3.1.3.72selective precipitation by heat and ammonium sulfate followed by anion exchange and chromatofocusing chromatography and gel filtration, purified 3300fold, to homogeneity 682641
Results 1 - 7 of 7