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Search Purification (Commentary)

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Results 1 - 6 of 6
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.67- 25140
Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.67fusion-protein is purified by Glutathione-Sepharose-4B affinity chromatography 693117
Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.67Poly-His tag proteins are purified with a HisTrap HP kit using buffers with 10 mM mercaptoethanol, enzymes are further purified with a gel filtration column in 100 mM NaCl, 10 mM Tris, pH 7.4, and 1 mM dithiothreitol. Final purification is done with a anion exchange column in 10 mM Tris, pH 7.4 with a linear gradient from 50-600 mM NaCl. 690927
Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.67recombinant enzyme 650767
Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.67recombinant PTEN is purified to near homogeneity using four sequential column chromatographic steps: a diethylaminoethyl (DEAE) Sepharose anion exchange column, a bio-gel hydroxyapatite HT (HAP) column, a Mono-S cation exchange column, and a Mono-Q anion exchange column 682679
Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.67treatment with alkaline phosphatase fully dephosphorylates the phosphorylation sites. Unphosphorylated PTEN and alkaline phosphatase can be separated by ion exchange column chromatography 682679
Results 1 - 6 of 6