EC Number   |
|---|
   3.1.3.53 | - |
| 3.1.3.53 | by immunoprecipitation from primary thymocytes using anti-PP1a, anti-PP1b, anti-MYPT1 antibodies in combination with protein A-Sepharose slurry, myosin phosphatase subunits PP1b and MYPT1 coimmunoprecipitate with HDAC7 |
| 3.1.3.53 | Flag MYPT1 using anti-Flag affinity chromatography, His tagged proteins by Ni-NTA chromatography |
| 3.1.3.53 | GSH affinity chromatography |
| 3.1.3.53 | immobilized metal ion affinity chromatography (Ni2+) |
| 3.1.3.53 | KAMPPase, copurification of MLCPase and myosin light chain kinase activities, forming a multienzyme complex |
| 3.1.3.53 | native enzyme 340fold by ion exchange and hydrophobic interaction chromatography |
| 3.1.3.53 | native holoenzyme |
| 3.1.3.53 | Ni2+-NTA-agarose column chromatography |
| 3.1.3.53 | of the native and recombinant proteins by immunoprecipitation |
