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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.1.25.1-
Display the word mapDisplay the reaction diagram Show all sequences 3.1.25.1100-300fold, extensive purification, attempts to concentrate the enzyme preparation by Aquacide II or Sephadex G50 powder results in loss of activity, UV-DNA-cellulose chromatography, hydroxyapatite chromatography, gel filtration through Sephadex or CM-Sephadex as well as sedimentation in sucrose density gradients results in loss of activity if used after phosphocellulose chromatography, enzyme is more stable in fractions containing large amounts of protein like bovine serum albumin
Display the word mapDisplay the reaction diagram Show all sequences 3.1.25.13500fold
Display the word mapDisplay the reaction diagram Show all sequences 3.1.25.14100fold, to homogeneity
Display the word mapDisplay the reaction diagram Show all sequences 3.1.25.1native enzyme
Display the word mapDisplay the reaction diagram Show all sequences 3.1.25.1recombinant enzyme
Display the word mapDisplay the reaction diagram Show all sequences 3.1.25.1recombinant from Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 3.1.25.1recombinant UvrA, UvrB, and UvrC
Display the word mapDisplay the reaction diagram Show all sequences 3.1.25.1recombinant wild-type and mutant C-terminally His-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel chelating affinity chromatography to homogeneity
Display the word mapDisplay the reaction diagram Show all sequences 3.1.25.1T127M
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