EC Number |
Reference |
---|
2.8.1.12 | recombinaant MoaB from Escherichia coli strain BL21(DE3) by ultracentrifugation, hydrophobic interaction chromatography, gel filtration, anion and cation exchange chromatography, an hydroxyapatite chromatography |
721182 |
2.8.1.12 | recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, and gel filtration |
723255 |
2.8.1.12 | recombinant fully activated MoaD, MoaE, and the E141DELTA variant of MoaE |
722627 |
2.8.1.12 | recombinant His-tagged wild-type and mutant protein from Escherichia coli strain M15 by nickel affinity chromatography and gel filtration. Elution of carboxylated or thiocarboxylated protein is induced by using a cleavage buffer containing 20 mM Tris/HCl, 500 mM NaCl, 0.1 mM EDTA, pH 8.0, with either 30 mM dithiothreitol or 30 mM ammonium sulfide, respectively. The cleavage reaction is performed at 4°C for at least 24 h |
722622 |
2.8.1.12 | recombinant His6-tagged MoaE and MoaD from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and gel filtration |
721609 |
2.8.1.12 | recombinant human subunits MOCS2A and MOCS2B from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and gel filtration. The separately purified subunits readily assemble into a functional MPT synthase tetramer |
722634 |
2.8.1.12 | recombinant intein-fusion wild-type and mutant MoaDs and MoaEs from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, gel filtration, and chitin affinity chromatography, intein cleavage, followed by another step of gel filtration |
722628 |