EC Number |
---|
2.7.1.48 | - |
2.7.1.48 | ammonium sulfate precipitation, Toyopearl Phenyl-650 M column chromatography, Toyopearl SP-650 column chromatography and Superdex 200 gel filtration |
2.7.1.48 | ammonium sulfate, Pb2+-precipitation |
2.7.1.48 | DE-52, ammonium sulfate, ATP-agarose column |
2.7.1.48 | DEAE-cellulose, ammonium sulfate, hydroxyapatite |
2.7.1.48 | immobilized metal ion adsorption chromatography (Ni2+) |
2.7.1.48 | Ni-NTA agarose column chromatography and Sephadex G25 gel filtration |
2.7.1.48 | partial purification |
2.7.1.48 | protamine sulfate, CM-cellulose, ammonium sulfate, DEAE-cellulose, zone electrophoresis |
2.7.1.48 | recombinant C-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography followed by ion-exchange chromatography |