EC Number   |
|---|
    2.7.1.26 | - |
| 2.7.1.26 | affinity chromatography |
| 2.7.1.26 | ammonium sulfate precipitation, phenyl Sepharose column chromatography, and DEAE-cellulose column chromatography |
| 2.7.1.26 | partial |
| 2.7.1.26 | partial, affinity chromatography |
| 2.7.1.26 | presence of ATP:riboflavin 5'-phosphotransferase and ATP:FMN adenylyltransferase on a single polypeptide |
| 2.7.1.26 | recombinant C-terminally poly-His-tagged N-terminally truncated mutant_187-338 from Escherichia coli by ion exchange chromatography and gel filtration |
| 2.7.1.26 | recombinant enzyme |
| 2.7.1.26 | recombinant His-tagged enzyme RFK from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by a protease, followed by ammonium sulfate fractionation, hydrophobic interaction chromatography, and dialysis. HsRFK is purified free of flavin and adenine ligands |
| 2.7.1.26 | recombinant RFK module of enzyme FADS, DELTA(1-182)CaFADS, from Escherichia coli strain BL21(DE3) |
