EC Number   |
|---|
    2.7.1.17 | - |
| 2.7.1.17 | apo-enzyme and in complex with D-xylulose, ATP plus D-xylulose or 5-deoxy-5-fluoro-D-xylulose, nickel affinity column chromatography and Superdex 200 gel filtration |
| 2.7.1.17 | harvested cells resuspended in 50 mM Tris/HCl pH 7.8, solution after ultrasonically disruption centrifuged at 10000 *g for 30 min at 4°C, supernatant passed through immobilized metal affinity chromatography (5 ml) using the His-tag introduced into the C-terminal region of XK, purified enzyme dialyzed against 50 mM Tris/Hcl (pH 7.8) containing 12.5% (w/v) glycerol |
| 2.7.1.17 | Ni-NTA column chromatography and Sephacryl S200 gel filtration |
| 2.7.1.17 | nickel affinity column chromatography and Superdex 200 gel filtration |
| 2.7.1.17 | partial |
| 2.7.1.17 | partial purification using Sepharose-Q column chromatography |
| 2.7.1.17 | recombinant enzyme |
