EC Number   |
Reference |
|---|
    2.7.1.156 | - |
644435, 644436, 644437, 644438 |
| 2.7.1.156 | CobU protein is overproduced and purified. Cells from a 4-liter culture of the overproducing strain are lysed using a French press. After the addition of ammonium sulfate, fast protein liquid chromatography is used to isolate CobU protein from the clarified cell extract in two additional steps, hydrophobic interaction chromatography and dye ligand chromatography. Protein concentration was determined by the Bradford method. |
674340 |
| 2.7.1.156 | recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, hydrophobic interaction chromatography on a phenyl resin, and ultrafiltration |
663316 |
