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Search Purification (Commentary)

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Results 1 - 10 of 13 > >>
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.6- 638040, 638041, 638046, 704429, 736587
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.6ammonium sulfate precipitation and Sephacryl S-200 gel filtration 737137
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.6Bio-Scale Mini UNOsphere Q column chromatography, and Superose 12 gel filtration 736846
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.6DRTase I and DRTAse II 638051
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.6preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20°C in 50% glycerol 691053
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.6recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 759063
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.6recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 759340
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.6recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and preparative gel fitration 758681
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.6recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and tag cleavage through thrombin 758927
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.6recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, method optimization for LaNDT 759530
Results 1 - 10 of 13 > >>