EC Number   |
Reference |
|---|
   2.4.2.2 | - |
489698, 638532, 639637, 639639, 639640, 639641 |
| 2.4.2.2 | harvested with 5 mM 2-mercaptoethanol and 500 mM NaCl in Tris-HCl pH 8, extract desalted with HiPrep 2610, recombinant protein eluted with 0-300 mM NaCl from Super Q Toyopearl 650M column, eluted from Bio-Scale CHT-20-1 column with 10-100 mM NaCl in phosphate-NaOH pH 7, concentrated by ultracentrifugation with 10 kDa cut-off, eluted from HiLoad 16/60 Superdex 200 prep-grade column with 200 mM NaCl in Tris-HCl pH 8, concentrated by ultracentrifugation to 21.7 mg/ml, stored at -70 °C |
677372 |
| 2.4.2.2 | recombinant enzyme from Escherichia coli strain BL21(DE3) by dialysis, anion exchange chromatography, and gel filtration |
758699 |
| 2.4.2.2 | recombinant enzymes |
659906 |
| 2.4.2.2 | recombinant N-terminally His6-tagged Py-NPase from Escherichia coli strain BL21 by nickel affinity chromatography |
760077 |
| 2.4.2.2 | recombinnat His-tagged enzyme by nickel affinity chromatography |
758599 |
