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EC Number
Purification (Commentary)
Reference
2.4.2.17
-
637665
,
637666
,
637670
,
637671
,
736541
,
758921
2.4.2.17
ammonium sulfate precipitation and DEAE-cellulose chromatography
637661
2.4.2.17
centrifugation and DEAE-Sephacel anion-exchange column
637674
2.4.2.17
chromatography on Sephadex G-75
637663
2.4.2.17
first purification by heating the crude extract, ammonium sulfate precipitation and selective histidine-dependent ammonium sulfate precipitation
637669
2.4.2.17
HisTrap column chromatography
758806
2.4.2.17
HisTrap resin column chromatography and Superdex S200 gel filtration
758758
2.4.2.17
HiTrap Talon column chromatography and Superdex 200 gel filtration
737208
2.4.2.17
HiTrap Talon resin column chromatography, GST-affinity column chromatography and Superdex S200 gel filtration
758757
2.4.2.17
immobilized metal ion affinity chromatography (Ni2+)
721673
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