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EC Number
Purification (Commentary)
Reference
2.4.1.87
-
707367
,
708606
2.4.1.87
calf
489466
2.4.1.87
centrifugation of cell culture, pellet lysed with EasyLyse, loaded onto Ni-chelate column, washing and elution with 20 mM Tris-HCl, pH 7.9, containing NaCl and imidazole
708604
2.4.1.87
different detergents for solubilization, best with Triton CF-54
489462
2.4.1.87
Ehrlich ascites carcinoma cells
489463
,
489471
2.4.1.87
HisTrap HP column chromatography
721667
2.4.1.87
partial
489462
,
489463
,
489465
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