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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.4- 489135, 489144, 489145, 672511, 756275, 756344, 756940, 757357, 757420
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.4Ni-NTA chromatography 756626
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.4nickel affinity chromatography 757037
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.4recombinant C-terminally His6-tagged enzyme by nickel affinity chromatography 720211
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.4recombinant enzyme 489140, 489141
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.4recombinant enzyme from Escherichia coli strain BL21 to homogeneity using GST-affinity chromatography followed by dialysis and subsequent tag removal via PreScission protease and reverse affinity chromatography 735389
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.4recombinant GST-tagged ASase from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin 721111
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.4recombinant GST-tagged DgAS by glutathione affinity chromatography, followed by proteolytic removal of the GST-tag 720052
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.4recombinant GST-tagged DGAS from Escherichia coli by glutathione affinity chromatography 719376
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.4recombinant GST-tagged DGAS from Escherichia coli strain BL21 by glutathione affinity chromatography 703057, 705197
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