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Search Purification (Commentary)

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Results 1 - 5 of 5
EC Number Purification (Commentary) Reference
Show all pathways known for 2.4.1.226Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.226- 488969
Show all pathways known for 2.4.1.226Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.226a soluble form of CSGlcA-T is generated by replacing the first 57 amino acids of the protein with a cleavable insulin signal sequence and a protein A IgG-binding domain. The soluble protein is expressed in COS-1 cells as a recombinant protein fused with the protein A IgG-binding domain. The fusion protein secreted into the medium is adsorbed onto IgG-Sepharose beads for purification to eliminate endogenous glycosyltransferases, and then the protein-bound beads are used as an enzyme source 690806
Show all pathways known for 2.4.1.226Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.226partially 488968
Show all pathways known for 2.4.1.226Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.226recombinant FLAG-tagged soluble catalytic domain from COS-7 cells 659234
Show all pathways known for 2.4.1.226Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.226recombinant soluble N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 758683
Results 1 - 5 of 5