Leibniz Institute DSMZ
DSMZ Digital Diversity
Login
Classic view
All enzymes
Enzyme history
BRENDA support
Any feedback?
Please rate this page
(search_result.php)
😁
😐
😡
(
0
/150)
Send feedback
BRENDA support
Refine search
Search Purification (Commentary)
Purification (Commentary):
show
10
50
100
results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search
Recommended Name:
EC Number:
contains
exact
begins with
ends with
use * as joker
Organism
:
contains
exact
begins with
ends with
use * as joker
Reference:
contains
exact
begins with
ends with
use * as joker
Search term:
Results
1
-
5
of
5
download as CSV
download all results as CSV
EC Number
Purification (Commentary)
Reference
2.4.1.226
-
488969
2.4.1.226
a soluble form of CSGlcA-T is generated by replacing the first 57 amino acids of the protein with a cleavable insulin signal sequence and a protein A IgG-binding domain. The soluble protein is expressed in COS-1 cells as a recombinant protein fused with the protein A IgG-binding domain. The fusion protein secreted into the medium is adsorbed onto IgG-Sepharose beads for purification to eliminate endogenous glycosyltransferases, and then the protein-bound beads are used as an enzyme source
690806
2.4.1.226
partially
488968
2.4.1.226
recombinant FLAG-tagged soluble catalytic domain from COS-7 cells
659234
2.4.1.226
recombinant soluble N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
758683
Results
1
-
5
of
5
download as CSV
download all results as CSV