EC Number   |
|---|
    2.3.1.179 | - |
| 2.3.1.179 | immobilized metal ion affinity chromatography (Ni2+) |
| 2.3.1.179 | Ni-NTA agarose column chromatography and HiLoad GF200 gel filtration |
| 2.3.1.179 | Ni-NTA Sepharose column chromatography and Superdex S200 gel filtration |
| 2.3.1.179 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinty chromatography, tag cleavage by TEV protease, gel filtration, and ultrafiltration to over 90% purity |
| 2.3.1.179 | recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinit chromatography, tag cleavage through TEV, dialysis, and gel filtration |
| 2.3.1.179 | recombinant wild-type and mutant His-tagged FabF from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 2.3.1.179 | to homogeneity (>9000-fold purification), ion exchange chromatography (CM-Sepharose, HR-DEAE), hydroxyapatite and affinity chromatography (ACP-Sepharose) |
