EC Number   |
|---|
    2.3.1.169 | - |
| 2.3.1.169 | native enzyme by anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography, gel filtration, and CoA affinity chromatography |
| 2.3.1.169 | recombinant ACSCh732 and ACSChDELTAN from Escherichia coli strain NM522 by anion exchange and hydroxyapatite chromatography, followed by another and different step of anion exchange chromatography and by hydrophobic interaction chromatography to 94-95% purity forACSCh and around 98% purity for ACSChDELTAN |
| 2.3.1.169 | recombinant ACSD beta from Escherichia coli strain NM522 by anion exchange chromatography, followed by hydrophobic interaction chromatography and another and different step of ion exchange of anion exchange chromatography to around 98% purity for ACDS beta |
| 2.3.1.169 | recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography under a N2 atmosphere |
