2.3.1.165 | recombinant N-terminally His6-tagged ATX and its thioester hydrolase domain, and the truncated mutant enzyme form, THID, from Escherichia coli strain BL21(DE3)-CodonPlus RIPL, by nickel affinity chromatography, and THID further by anion exchange chromatography, followed by gel filtration |
719864 |