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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.164- 441307
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.164cell extracts of Penicillium chrysogenum are prepared, Escherichia coli soluble extracts are used for in vitro IAT activity measurement 686918
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.164recombinant wild-type enzyme with a C-terminal His-tag is purified in a single step using Ni2+ affinity chelate chromatography. A combination of 5 mM DTT + 250 mM NaCl + 5 mM EDTA is effective in preventing aggregation of the enzyme 660448
Results 1 - 3 of 3