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Search Purification (Commentary)

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Results 1 - 5 of 5
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.122ammonium sulfate fractionation, gel filtration and Sephadex column chromatography 486242
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.122native enzyme from strain BCG, recombinant enzyme from Escherichia coli 673960
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.122recombinant proteins purified by affinity chromatography on Ni2+ charged His-trap columns 486244
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.122using Ni-NTA chromatography 702229, 704439
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.122using Ni-NTA chromatography and by applying a linear imidazole gradient from 10 to 500 mM. The final purification step contains gel filtration on a Superdex 200 column 705100
Results 1 - 5 of 5