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EC Number
Purification (Commentary)
Reference
2.3.1.122
ammonium sulfate fractionation, gel filtration and Sephadex column chromatography
486242
2.3.1.122
native enzyme from strain BCG, recombinant enzyme from Escherichia coli
673960
2.3.1.122
recombinant proteins purified by affinity chromatography on Ni2+ charged His-trap columns
486244
2.3.1.122
using Ni-NTA chromatography
702229
,
704439
2.3.1.122
using Ni-NTA chromatography and by applying a linear imidazole gradient from 10 to 500 mM. The final purification step contains gel filtration on a Superdex 200 column
705100
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