EC Number   |
Reference |
|---|
   2.1.1.260 | native enzyme partially from subcellular fractions by sucrose density gradient fractionation |
721118 |
| 2.1.1.260 | recombinant His-tagged Nep1 from Escherichia coli strain M15(pREP4) by nickel affinity chromatography |
720582 |
| 2.1.1.260 | recombinant His6-tagged Nep1 from Escherichia coli strain RosettaTM-DE3-pLysS by nickel affinity chromatography, cleavage of the tag by TEV protease, and two steps of gel filtration to over 99% purity |
720589 |
| 2.1.1.260 | recombinant N-terminally His6-tagged wild-type and D90G mutant Nep1 from Escherichia coli strain XL1-blue by nickel affinity chromatography and gel filtration |
720587 |
| 2.1.1.260 | recombinant Nep1 by cation exchange chromatography and gel filtration |
720582 |
| 2.1.1.260 | recombinant Nep1p from Escherichia coli strain BL21(DE3) by ion exchange chromatography and gel filtration |
720579 |
| 2.1.1.260 | recombinant wild-type and selenomethionine-labeled His6-tagged Nep1 from Escherichia coli strain RosettaTM-DE3-pLysS by nickel affinity chromatography, cleavage of the tag by TEV protease, and two steps of gel filtration to over 99% purity |
720589 |
