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Search Purification (Commentary)

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Results 1 - 10 of 17 > >>
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.228- 661154, 662622, 673850, 711197, 712737, 729858
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.228recombinant C-terminally His-tagged enzyme, lacking the N-terminal peptide V2LWILWRP9, from Escherichia coli by metal ion affinity chromatography and anion exchange chromatography 737226
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.228recombinant enzyme 712103
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.228recombinant expression of His-tagged enzyme by affinity chromatography and desalting gel filtration 758324
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.228recombinant expression of His-tagged wild-type and mutant enzymes to near homogeneity by 20 min heat treatment at 70°C and 65°C, respectively, followed by affinity chromatography and desalting gel filtration 758324
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.228recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography 757863
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.228recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography 737228
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.228recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through 3C protease, another step of nickel affinity chromatography, followed by gel filtration 757408
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.228recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration 756152
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.228recombinant His-tagged isolated N-terminal domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration 756153
Results 1 - 10 of 17 > >>