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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the reaction diagram Show all sequences 2.1.1.218-
Display the reaction diagram Show all sequences 2.1.1.218recombinant His-tagged full-length wild-type enzyme, truncated enzyme SaTrm10 1-249, and enzyme mutants from Escherichia coli strain Rosetta (DE3)pLysS by nickel affinity chromatography and gel filtration, recombinant nontagged wild-type enzyme by anion exchange and cation exchange chromatography, followed by gel filtration
Display the reaction diagram Show all sequences 2.1.1.218recombinant N-terminally His6-tagged protein from Escherichia coli by nickel affinity chromatography and dialysis
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