EC Number   |
|---|
   1.8.99.B2 | - |
| 1.8.99.B2 | 71fold enrichment with a recovery of 4.3% of the total activity |
| 1.8.99.B2 | native enzyme 71.4fold from the membrane fraction of strain SH by solubilization with n-dodecyl-beta-D-maltopyranoside, ultracentrifugation, anion exchange chromatography, dialysis, ultrafiltration, and gel filtration |
| 1.8.99.B2 | native enzyme 71.4fold from the solubilized membrane fraction by ultracentrifugation, anion exchange chromatography, dialysis, and ultrafiltration |
| 1.8.99.B2 | native periplasmic enzyme from strain ATCC 23270 and recombinant enzyme from Escherichia coli strain BL21(DE3) celll extract by ammonium sulfate fractionation, cation exchange chromatography, dialysis, hydrophobic interaction chromatography, ultrafilatration, and gel filtration |
| 1.8.99.B2 | recombinant Strep-tagged enzyme TsdA 41fold from Escherichia coli strains BL21(DE3) and C43(DE3) by membrane solubilization, affinity chromatography, desalting gel filtration, and ultrafiltration recombinant Strep-tagged TsdC by two steps of ultracentrifugation at 100000 x g, followed by affinity chromatography |
| 1.8.99.B2 | using chromatography on Phenyl-Sepharose column, CM-cellulose column and hydroxylapatite column |
