EC Number |
---|
1.8.7.1 | - |
1.8.7.1 | ammonium sulfate precipitacion, filtration on a superdex-75 column |
1.8.7.1 | cell extract is treated with DEAE-cellulose in a batchwise manner, the unbound fraction is fractionated by ammonium sulfate precipitation, anion-exchange and hydrophobic column chromatography are applied |
1.8.7.1 | Ni2+-nitrilotriacetic acid affinity matrix and Q-Sepharose column chromatography |
1.8.7.1 | partially, crude leaves extract |
1.8.7.1 | phenyl-sepharosa column, DNA cellulose column |
1.8.7.1 | phenyl-Sepharose chromatography, F420-Sepharose chromatography and QAE-Sephadex gel filtration |
1.8.7.1 | recombinant His-tagged wild-type and mutant enzymes, as well as enzyme CysG, by alternating ultrafiltration and nickel affinity chromatography, followed by anion exchange chromatography, ultrafiltration, and gel filtration |