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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.B1-
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.B12',5'-ADP Sepharose column chromatography and Sephadex G-25 gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.B1ADP-agarose column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.B1ammonium sulfate precipitation, Ni-NTA column chromatography, GSH-Sepharose column chromatography, and 2',5'-ADP-Sepharose column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.B1DEAE-Sephacel gel filtration, hydroxyapatite chromatography column chromatography, and Cibacron blue chromatography column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.B1Ni-NTA column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.B1nickel affinity column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.B1recombinant enzyme
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.B1recombinantly produced human enzyme (hTGR), the Sec->Cys mutant (hTGRU642C) of the full-length protein, the isolated TrxR domain (hTGR151-643) and the Grx domain containing a monothiol active site (hTGR1-150) are produced
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.B1small amounts of the soluble enzyme are obtained by purifying it from a large quantity of cells, in which protein synthesis is induced slowly for a period of 24 h at a low temperature
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