EC Number   |
|---|
   1.7.2.2 | - |
| 1.7.2.2 | native enzyme 57.7fold by anion exchange chromatography and gel filtration |
| 1.7.2.2 | non-denaturing preparative polyacrylamide gel electrophoresis |
| 1.7.2.2 | purification by sepharose fast flow chromatography and gel filtration |
| 1.7.2.2 | Q-Sepharose HP column chromatography, Superdex S200 gel filtration and S-200 HR column chromatography |
| 1.7.2.2 | recombinant MBP-tagged enzyme from Wolinella succinogenes strain DSM 1740 by affinity chromatography, cleavage of the MBP-tag by TEV protease |
| 1.7.2.2 | recombinant protein, by Strep-tag II affinity chromatography followed by size-exclusion chromatography |
| 1.7.2.2 | recombinant wild-type and mutant enzymes from Escherichia coli strain JCB4083a by ammonium sulfate fractionation and anion exchange chromatography |
| 1.7.2.2 | recombinant wild-type and mutant MBP-tagged enzymes from Wolinella succinogenes strain DSM 1740 by affinity chromatography, cleavage of the MBP-tag by TEV protease |
