EC Number   |
|---|
   1.7.1.3 | ammonium slufate precipitation, ion-exchange, gel filtration, hydroxylapatite column. FAD and EDTA essential in all buffers during purification |
| 1.7.1.3 | ammonium sulfate precipitation |
| 1.7.1.3 | gel filtration |
| 1.7.1.3 | immobilized metal affinity chromatography and SourceQ15 column anion exchange chromatography |
| 1.7.1.3 | ion-exchange, ammonium sulfate precipitation, gel filtration |
| 1.7.1.3 | metal-chelate affinity chromatography for his-tagged proteins, ammonium sulfate fractionation, affinity chromatography |
| 1.7.1.3 | native enzyme 70fold by ultrafiltration, anion exchange chromatography, and gel filtration |
| 1.7.1.3 | partially purification of wild-type and nit-3 mutant by streptomycin sulfate precipitation and ammonium sulfate fractionation, of nit-1 mutant by protamine sulfate fractionation, ammonium sulfate precipitation and gel filtration |
| 1.7.1.3 | partially purified |
| 1.7.1.3 | protamine sulfate and ammonium sulfate precipitation, hemoglobin-Sepharose column, Bio-Gel A, FAD-Sepharose affinity column. proteolysis during extensive purification |
