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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the reaction diagram Show all sequences 1.5.99.B4recombinant His- and S-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography as holoenzymes. The expression level of the alphaC382Sbetagamma mutant is approximately 6fold lower than that of alphabetagamma-wild-type
Display the reaction diagram Show all sequences 1.5.99.B4recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration
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