EC Number   |
|---|
   1.5.99.15 | - |
| 1.5.99.15 | HisTrap column chromatography and S200 gel filtration |
| 1.5.99.15 | recombinant His6-tagged enzyme from Escherichia col strain BE100 [BL21(DE3) RIL] in an anaerobic chamber by heat treatement at 65°C for 5 min, the supernatant is a second time heat treated at 85°C for 15 min, |
| 1.5.99.15 | recombinant His6-tagged enzyme from Escherichia coli strain BE100 [BL21(DE3) RIL] in an anaerobic chamber by nickel affinity chromatography, ultrafiltration, and gel filtration |
| 1.5.99.15 | recombinant N-terminally His6 -tagged enzyme from Escherichia coli strain BL21DE3 RIL cell-free extract by nickel affinity chromatography and gel filtration |
