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Search Purification (Commentary)

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Results 1 - 10 of 16 > >>
EC Number Purification (Commentary) Reference
Show all pathways known for 1.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 1.5.3.1- 392376, 392377, 392378, 392380, 392384, 742678
Show all pathways known for 1.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 1.5.3.1affinity matrix is 5-formyltetrahydrofolate coupled to AH-sepharose 392394
Show all pathways known for 1.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 1.5.3.1affinity-purification, 4-aminopyrrole-2-carboxylic acid as the affinity ligand, which is coupled with Sepharose CL 4B via spacers composed of epichlorohydrin and ethylenediamine. With the affinity medium, the purification process consists of only one affinity chromatography step to capture monomeric enzyme. The purified enzyme iss 94% and 96% pure when analyzed on an HPLC and reducing SDS-PAGE 743178
Show all pathways known for 1.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 1.5.3.1DEAEcellulose, QAE-Sephadex, and Ultrogel column chromatographies 764086
Show all pathways known for 1.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 1.5.3.1HiTrap DEAE column chromatography, HisTrap column chromatography, HiTrap phenyl column chromatography, Resources Q column chromatography, and Superdex 200 gel filtration 764062
Show all pathways known for 1.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 1.5.3.1native enzyme by ammoniumm sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration 724775
Show all pathways known for 1.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 1.5.3.1P-1 392391
Show all pathways known for 1.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 1.5.3.1recombinant His-tagged alpha and beta subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity 724937
Show all pathways known for 1.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 1.5.3.1recombinant native and mutant enzyme 685219
Show all pathways known for 1.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 1.5.3.1recombinant protein 671547
Results 1 - 10 of 16 > >>