EC Number   |
|---|
    1.5.3.1 | - |
| 1.5.3.1 | affinity matrix is 5-formyltetrahydrofolate coupled to AH-sepharose |
| 1.5.3.1 | affinity-purification, 4-aminopyrrole-2-carboxylic acid as the affinity ligand, which is coupled with Sepharose CL 4B via spacers composed of epichlorohydrin and ethylenediamine. With the affinity medium, the purification process consists of only one affinity chromatography step to capture monomeric enzyme. The purified enzyme iss 94% and 96% pure when analyzed on an HPLC and reducing SDS-PAGE |
| 1.5.3.1 | DEAEcellulose, QAE-Sephadex, and Ultrogel column chromatographies |
| 1.5.3.1 | HiTrap DEAE column chromatography, HisTrap column chromatography, HiTrap phenyl column chromatography, Resources Q column chromatography, and Superdex 200 gel filtration |
| 1.5.3.1 | native enzyme by ammoniumm sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration |
| 1.5.3.1 | P-1 |
| 1.5.3.1 | recombinant His-tagged alpha and beta subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity |
| 1.5.3.1 | recombinant native and mutant enzyme |
| 1.5.3.1 | recombinant protein |
