EC Number   |
|---|
   1.4.1.20 | - |
| 1.4.1.20 | best purification results of recombinant PheDH are achieved in an polymer/salt aqueous two-phase systems (ATPS) with 9% of phenazine ethosulfate with a molecular mass of 6000 Da (PEG-6000) and 16% of K2HPO4 |
| 1.4.1.20 | construction of a chimeric enzyme consisting of the N-terminal domain of Thermoactinomyces intermedius phenylalanine dehydrogenase, containing the substrate-binding region and the C-terminal domain of leucine dehydrogenase from Bacillus stearothermophilus containing the NAD+-binding region |
| 1.4.1.20 | in aqueous two-phase systems composed of polyethylene glycol 6000 and ammonium sulfate with 8.5% (w/w) PEG-6000, 17.5% (w/w) (NH4)2SO4 and 13% (w/w) NaCl at pH 8.0 |
| 1.4.1.20 | mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q |
| 1.4.1.20 | Ni-affinity and anion exchange column chromatography |
| 1.4.1.20 | Ni-affinity column chromatography |
| 1.4.1.20 | Ni-affinity column chromatography and anion exchange column chromatography |
| 1.4.1.20 | Ni-IDA column chromatography |
| 1.4.1.20 | partial |
