EC Number   |
|---|
    1.4.1.16 | - |
| 1.4.1.16 | ammonium sulfate, DEAE-cellulose, hydroxyapatite, Sephadex G-150 |
| 1.4.1.16 | Ni+-charged chelating Sepharose column chromatography and Toyopearl butyl-650M column chromatography |
| 1.4.1.16 | Ni-NTA column chromatography |
| 1.4.1.16 | Ni-NTA column chromatography and Superdex 200 gel filtration |
| 1.4.1.16 | Ni-NTA column chromatography, and Superdex 200 gel filtration |
| 1.4.1.16 | recombinant enzyme from Escherichia coli strain Rosetta (DE3) by heat treatment and affinity chromatography, native enzyme 47fold to homogeneity by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography, followed by preparative slab PAGE |
| 1.4.1.16 | recombinant protein |
| 1.4.1.16 | recombinant soluble enzyme 3.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 1.4.1.16 | recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) |
