EC Number   |
|---|
   1.3.7.3 | - |
| 1.3.7.3 | by gel filtration |
| 1.3.7.3 | by two affinity chromatography steps to 90% purity and a third gel filtration step |
| 1.3.7.3 | GST-tagged PebA forms inactive aggregates |
| 1.3.7.3 | recombinant GST-tagged or His-tagged PebB from Escherichia coli BL21(DE3) by glutathione or nickel affinity chromatography, gel filtration, and ultrafiltration, recombinant tagged PebAgB by affinity chromatography |
| 1.3.7.3 | recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli by DNA removal from cell extract, ultracentrifugation at 220000 x g, glutathione affinity chromatography and cleavage of the tag through TEV protease, followed by dialysis and a second step of glutathione affinity chromatography to remove that tag, gel filtration, and ultrafiltration |
