EC Number |
---|
1.3.3.5 | - |
1.3.3.5 | ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex-G-100 gel filtration |
1.3.3.5 | ammonium sulfate, charcoal, Sephadex a-50, Sephadex G-100 |
1.3.3.5 | by gel filtration |
1.3.3.5 | DEAE-Sepharose Fast Flow column chromatography and Sephadex G-100 gel filtration |
1.3.3.5 | extracellular enzyme 3.92fold by ammonium sulfate fractionation and anion exchange chromatography, an additional step of gel filtration lowers the enzyme purity and amount |
1.3.3.5 | native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration |
1.3.3.5 | native enzyme by anion exchange chromatography and hydrophobic interaction chromatography |
1.3.3.5 | Ni-NTA column chromatography |
1.3.3.5 | Q sepharose column chromatography |