EC Number   |
|---|
   1.3.1.91 | - |
| 1.3.1.91 | HiTrap column chromatography and Superdex S200 gel filtration |
| 1.3.1.91 | Ni-NTA column chromatography and Superdex 200 gel filtration |
| 1.3.1.91 | Ni-NTA column chromatography and Superdex S200 gel filtration |
| 1.3.1.91 | recombinant enzyme |
| 1.3.1.91 | recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration |
| 1.3.1.91 | recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli cell-free extract (centrifugation at 40000 x g) by nickel affinity chromatography, dialysis, heparin affinity chromatography, and gel filtration |
| 1.3.1.91 | recombinant wild-type and selenomethionine-labeled hDus2 1-340 fragment, comprising the hDus2 catalytic and tRNA-recognition domains, from Escherichia coli strains BL21(DE3) and B834(DE3), respectively |
