EC Number |
---|
1.3.1.124 | - |
1.3.1.124 | affinity chromatography |
1.3.1.124 | gel filtration, DEAE-cellulose column chromatography, affinity chromatography |
1.3.1.124 | ion-exchange and affinity chromatography |
1.3.1.124 | metal ion affinity chromatography |
1.3.1.124 | partial |
1.3.1.124 | purification and separation of the peroxisomal and mitochondrial 2,4-dienoyl-CoA reductases |
1.3.1.124 | purification via dye-ligand chromatography using immobilized blue Sepharose CL-6B and Matrex gel red A as matrices and general ligand chromatography employing 2',5'-ADP-Sepharose 4B and 3',5'-ADP-agarose |
1.3.1.124 | recombinant His-tagged truncated wild-type and of mutant enzymes from Escherichia coli strain BL21(DE3) by one-step nickel affinity chromatography, to over 95% purity |
1.3.1.124 | recombinant protein, one-step purification with a nickel HiTrap chelating metal affinity column |