EC Number   |
|---|
   1.2.5.3 | native enzyme 13fold to homogeneity by ultracentrifugation, anion exchange chromatography, ultrafiltration, hydroxyapatite chromatography, again ultrafiltration, and gel filtration |
| 1.2.5.3 | native enzyme 21fold from cell-free extract by ultracentrifugation, anion exchange chroomatography, ultrafiltration, hydrophobic interaction chromatography, ultrafiltration, and gel filtration |
| 1.2.5.3 | native enzyme by ammonium sulfate fractionation, dialysis, and anion exchange chromatography, followed by ultrafiltration, and gel filtration |
| 1.2.5.3 | recombinant protein, purification by Ni-NTA and size exclusion chromatography |
| 1.2.5.3 | recombinant recombinant M subunit 8fold by ultracentrifugation, anion exchange chromatography, and gel filtration from Escherichia coli strain BL21(DE3) |
