EC Number   |
|---|
    1.2.1.79 | - |
| 1.2.1.79 | nickel chelating Sepharose column chromatography and S200 16/60 gel filtration |
| 1.2.1.79 | recombinant enzyme |
| 1.2.1.79 | recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography and ultrafiltration, followed by tag cleavage through PreScission protease in a batch and the protease resin is eliminated by centrifugation |
| 1.2.1.79 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and dialysis, to homogeneity |
| 1.2.1.79 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration |
| 1.2.1.79 | recombinant N-terminally His-tagged SySSADH from Escherichia coli strain B834(DE3) by nickel affinity chromatography, dialysis and cleavge fo the His-tag by tobacco etch mosaic virus protease, followed by another step of immobilized metal affinity chromatography and gel filtration. Recombinant His-tagged enzyme mutants from Escherichia coli strain coli BL21(DE3) by immobilized metal affinity chromatography and dialysis |
| 1.2.1.79 | through Ni2+ affinity column chromatography, followed by a Hi-Load Superdex S-75 26/60 column chromatography |
| 1.2.1.79 | using immobilized metal affinity chromatography, removal of N-terminal His tag, further purification by immobilized metal affinity chromatography and gel filtration using a Superdex 200 column |
