EC Number |
---|
1.2.1.42 | alcohol-generating and aldehyde-generating acyl-CoA reductases, Blue A agarose, gel filtration, hexadecanoyl-CoA affinity chromatography |
1.2.1.42 | amylose column chromatography, metal affinity column chromatography, and G25 Sephadex gel filtration |
1.2.1.42 | Blue A agarose, Sephacryl S-100, palmitoyl-CoA agarose |
1.2.1.42 | heating, ammonium sulfate, gel filtration |
1.2.1.42 | HisTrap column chromatography and Ni2+ resin column chromatography |
1.2.1.42 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography |
1.2.1.42 | solubilization from microsomal preparation with 20% glycerol and 3 M NaCl, affinity chromatography |