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EC Number
Purification (Commentary)
Reference
1.2.1.22
-
288131
,
288132
,
288133
,
288135
1.2.1.22
anion-exchange chromatography
674270
1.2.1.22
by gel filtration
675430
1.2.1.22
mutant F180T purified to homogeneity (more than 95% pure), on Ni2+ resin
695015
1.2.1.22
Ni-affinity chromatography
670833
1.2.1.22
nickel-chelating affinity column
697876
1.2.1.22
partial
288136
1.2.1.22
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange chromatography to near homogeneity
742119
1.2.1.22
three chromatography steps
675430
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