EC Number   |
|---|
    1.2.1.10 | - |
| 1.2.1.10 | ADHE cannot be solubilized from membrane with detergents such as 1% Triton X-100 or 1% sulfobetaine 3-12. The enzyme is easily dissociated from membrane by high-salt buffers containing either 1.0 M NaCl or (NH4)2SO4 without detergents |
| 1.2.1.10 | by affinity chromatography by means of a Ni-Sepharose column |
| 1.2.1.10 | from anaerobically grown cells, rod-shaped |
| 1.2.1.10 | native enzyme from chloroplast stroma by anion exchange chromatography and affinity chromatography, followed by ultracentrifugation |
| 1.2.1.10 | native enzyme from plasma membrane by ultracentrifugation, hydroxyapatite and hydrophobic interaction chromatography, followed by ultrafiltration and gel filtration. The enzyme cannot be solubilized from membrane with detergents, either 1% Triton X-100 or 1% sulfobetaine 3-12 |
| 1.2.1.10 | Ni-NTA column chromatography |
| 1.2.1.10 | on DEAE and NAD+-linked affinity columns, combined with an ammonium sulfate fractionation step |
| 1.2.1.10 | partial |
| 1.2.1.10 | Q-Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration |
