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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 1.14.13.59Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.59recombinant enzyme form IucD439, in which the sequence encoding the IucD protein is fused in frame to the amino-terminal sequence of beta-galactosidase 207906
Show all pathways known for 1.14.13.59Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.59recombinant His-tagged wild-type and P14G mutant enzymes from strain M15-2 to homogeneity by gel filtration, ion exchange and nickel affinity chromatography 658232
Show all pathways known for 1.14.13.59Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.59recombinant His8-MBP-tagged wild-type and mutant K184A enzymes, as well as seleno-L-methionine-labeled enzyme from Escherichia coli TOP10 cells by nickel affinity chromatography, dialysis, tag cleavage by TEV protease, followed by a second step of nickel affinity chromatography and by MBP-tracking affinity chromatography, eluting the detagged enzyme, followed by dialysis 745322
Show all pathways known for 1.14.13.59Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.59recombinant wild-type and mutants from strain DH5alpha 658171
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