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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the reaction diagram Show all sequences 1.14.13.196- 724289, 724451
Display the reaction diagram Show all sequences 1.14.13.196recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography, depending on the imidazole concentration used, the recombinant enzyme is isolated either in the apo (FAD-free) or holo (FAD-bound) form, cleavage of the tag by TEV protease 745824
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