EC Number |
---|
1.13.12.16 | - |
1.13.12.16 | DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography |
1.13.12.16 | DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography; recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography |
1.13.12.16 | HiLoad XK 16 Superdex 200 prep-grade column gel filtration and Mono Q HR5/5 column chromatography |
1.13.12.16 | HisTrap column chromatography |
1.13.12.16 | phenyl Sepharose column chromatography and DEAE Sepharose column chromatography |
1.13.12.16 | purified to homogeneity |
1.13.12.16 | recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography |
1.13.12.16 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta(DE3)pLysS by affinity chromatography |
1.13.12.16 | to homogeneity |