EC Number   |
|---|
   1.13.11.24 | - |
| 1.13.11.24 | DEAE-Sephacel column equilibrated with 50 mM Tris.HCl, pH 7.5, and eluted with a NaCl gradient (0-600 mM). Ultrogel ACA 34 column, eluted with 50 mM Tris.HCl, pH 7.5, and 100 mM Nacl. DEAE-Sepharose column equilibrated with 50 mM Tris.HCl, pH 7,5, eluted with a gradient of NaCl (100-500 mM). |
| 1.13.11.24 | metal chelate affinity chromatography |
| 1.13.11.24 | partial purification by ammonium sulfate precipitation and DEAE Toyopearl column chromatography |
| 1.13.11.24 | partially |
| 1.13.11.24 | recombinant C-terminally Strep-tagged wild-type and mutant enzymes from Escherichia coli to electrophoretic homogeneity by hydrophobic interaction chromatography, dialysis, two different steps of anion exchange chromatography, dialysis and ultrafiltration |
| 1.13.11.24 | recombinant QueD protein with a C-terminal hexahistidine-tag |
| 1.13.11.24 | separation of isozymes |
